Reminder: Data may be unstable during the site migration. Thank you for your understanding!
Global Site
Current template:PCR Kit Template
PCR Test Kit for Detecting Maize-Derived Components
Quantitative Real-Time PCRHigh Sensitivity
PCR DETECTION KIT

PCR Test Kit for Detecting Maize-Derived Components

Precise targeting of plant endogenous genes is critical for starch purity identification and GMO detection. This maize-derived component nucleic acid test kit utilizes real-time quantitative PCR (TaqMan probe) technology to specifically target the maize endogenous Adh1 gene. Compliant with multiple national and entry-exit standards (e.g., SN/T 5522.7 Starch Identification Standard, GB/T 19495.4 GMO Detection Standard), this product provides high-precision molecular evidence for food starch adulteration screening and ingredient traceability.

10 copies
Limit of Detection
40-45
Cyclic number
Get in Touch

Citation Standards

GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method
SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method
ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms
Technical Principles

Real-time PCR

Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.

Nucleic Acid Extraction

Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.

PCR amplification

Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.

Fluorescence Detection

The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.

Core Performance Metrics

Core Technical Specifications

Rigorous quality control system ensures stability and reliability for every product batch.

Limit of Detection (LOD)
0.01% (by mass)
Specificity
No cross-reactivity
Linear Range
10^1 - 10^8 copies/mL
Repeatability (CV)
≤3%
Reaction System
25 μL / 50 μL
Packaging Size
48T/box
Amplification Procedure

Standard Amplification Procedure

Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.

StepsTemperatureTimeCyclic NumberDescription
pre-deformation95°C3-5 min1Activate DNA polymerase
gender transition95°C10-15 sec40-45Collect fluorescence signal
Annealing/Extension55-60°C30-40 sec

Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.

Compatible Instruments

Compatible Instruments

Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs

ABI 7500 / 7500 Fast
LightCycler 480 II
CFX96 / CFX384
QuantStudio 5 / 6 / 7
Mx3000P / Mx3500P
Rotor-Gene Q

Storage Conditions

Reagent Storage
Store below 20°C in the dark. Valid for 12 months.
Shipping Conditions
Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.
Storage after reconstitution
2-8°C for stable storage of 7 days; long-term storage at -20°C

Important Notes

Not intended for clinical diagnosis.
Wear a lab coat and gloves during operation to prevent contamination.
Vortex all reagents thoroughly and briefly centrifuge prior to use.
Prepare the reaction mixture in a laminar flow hood or biosafety cabinet.
Product Details

Product Details

Core Advantages

Ultra-High Sensitivity, Pushing Limits: Detects corn nucleic acid residues as low as 0.01% (w/w) in highly processed foods and purified starches.

Optimized for starch traceability with ultimate specificity: Designed to address pain points in the starch and agricultural product markets. Rigorously validated to show no cross-reactivity with common sources of easily confused starches—such as sweet potato, cassava, potato, lotus root—and major crops like soybean and peanut. Accurately detects starch substitution and adulteration.

Built-in UNG enzyme anti-contamination system: The pre-mixed reagent includes a dUTP/UNG enzyme system that actively degrades potential PCR aerosol contamination in the lab environment, eliminating false positives at the source.

Superior cap stability: Robust reagent components maintain amplification performance after up to 8 hours of room-temperature exposure, enabling efficient handling of large-scale sample batches.


Downloads & Support

Downloads & Technical Support

A professional technical team providing comprehensive product application and testing solution support.

Need technical consultation?

Our technical expert team will provide you with professional product consulting and customized solutions.

Get Technical Consultation Now
Contact Us
  • Buildings F4-F6, Bio-Industry Community, Changyuan Road, Wuqing District, Tianjin
  • 400-0118-190
  • info@xinocler.com
2026 Sinotongke (Beijing) Biotechnology Co., Ltd.

Cookie Usage Notice

Xinotongke's official website uses cookies and related technologies to enhance your browsing experience, analyze site traffic, optimize service performance, and deliver personalized content. These include essential cookies (for basic site functionality), performance cookies (to analyze usage data), and functional cookies (to remember your preferences).

继续使用本网站即表示您同意我们按照《Cookie 政策》使用相关技术。您可以随时通过浏览器设置管理或禁用 Cookie。

Learn more