在食用淀粉纯度鉴定及转基因产品检测中，对植物内源基因的精准靶向至关重要。玉米源性成分核酸检测试剂盒基于实时荧光定量PCR（TaqMan探针法）技术，特异性靶向玉米的内源 Adh1 基因。本产品严格遵循多项国家及出入境标准（如SN/T 5522.7淀粉鉴别标准、GB/T 19495.4转基因检测标准），为食品淀粉掺假筛查及成分溯源提供高精度的分子级证据。
Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.
Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.
Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.
The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.
Rigorous quality control system ensures stability and reliability for every product batch.
Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.
| Steps | Temperature | Time | Cyclic Number | Description |
|---|---|---|---|---|
| pre-deformation | 95°C | 3-5 min | 1 | Activate DNA polymerase |
| gender transition | 95°C | 10-15 sec | 40-45 | Collect fluorescence signal |
| Annealing/Extension | 55-60°C | 30-40 sec |
Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.
Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs
超高灵敏度，挑战极限： 检出限（LOD）低至惊人的 0.01%（质量百分比），能够精准捕捉深加工食品及高度纯化淀粉中极微量的玉米核酸残留。
专为淀粉溯源优化，极致排他： 针对淀粉及农产品市场痛点设计。经严苛验证，与甘薯（红薯）、木薯、马铃薯、藕等常见易混淆淀粉源，以及大豆、花生等均无交叉反应，精准识破淀粉替换与掺假。
内置 UNG 酶防污染系统： 试剂预混液标配 dUTP/UNG 酶防污染体系，能够主动降解实验室环境中潜在的PCR气溶胶污染，从根源上杜绝假阳性。
卓越的开盖稳定性： 试剂组分抗逆性强，支持室温下开盖暴露长达 8 小时而扩增性能无显著降低，从容应对大批量样本的集中加样。
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