Current template:PCR Kit Template

SN Standard for Nucleic Acid Detection Kit of Sheep Origin

Quantitative Real-Time PCRHigh Sensitivity

PCR DETECTION KIT

SN Standard for Nucleic Acid Detection Kit of Sheep-Derived Ingredients

Authenticity verification and adulteration screening of mutton and related products are critical priorities in food safety regulation. This kit, based on real-time quantitative PCR (TaqMan probe method), specifically targets the COX I gene of sheep. Compliant with SN/T 2051-2008, a national entry-exit inspection and quarantine standard, it enables rapid, sensitive, and accurate qualitative analysis of sheep-derived components in samples.

10 copies

Limit of Detection

40-45

Cyclic number

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Citation Standards

GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method

SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method

ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms

Technical Principles

Real-time PCR

Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.

Nucleic Acid Extraction

Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.

PCR amplification

Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.

Fluorescence Detection

The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.

Core Performance Metrics

Core Technical Specifications

Rigorous quality control system ensures stability and reliability for every product batch.

Limit of Detection (LOD)

0.1% (by mass)

Specificity

No cross-reactivity

Linear Range

10^1 - 10^8 copies/mL

Repeatability (CV)

≤3%

Reaction System

25 μL / 50 μL

Packaging Size

48T/box

Amplification Procedure

Standard Amplification Procedure

Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.

Steps	Temperature	Time	Cyclic Number	Description
pre-deformation	95°C	3-5 min	1	Activate DNA polymerase
gender transition	95°C	10-15 sec	40-45	Collect fluorescence signal
Annealing/Extension	55-60°C	30-40 sec	40-45	Collect fluorescence signal

Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.

Compatible Instruments

Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs

ABI 7500 / 7500 Fast

LightCycler 480 II

CFX96 / CFX384

QuantStudio 5 / 6 / 7

Mx3000P / Mx3500P

Rotor-Gene Q

Storage Conditions

Reagent Storage

Store below 20°C in the dark. Valid for 12 months.

Shipping Conditions

Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.

Storage after reconstitution

2-8°C for stable storage of 7 days; long-term storage at -20°C

Important Notes

Not intended for clinical diagnosis.

Wear a lab coat and gloves during operation to prevent contamination.

Vortex all reagents thoroughly and briefly centrifuge prior to use.

Prepare the reaction mixture in a laminar flow hood or biosafety cabinet.

Product Details

Core Advantages

Authoritative compliance, highly reliable:Product development and performance metrics strictly comply with SN national standards and GB/T laboratory specifications. Manufactured under an ISO 13485 medical-grade quality management system, it meets the quality control requirements of professional testing agencies.

High Sensitivity and Strong Specificity:Limit of Detection (LOD) as low as0.1%(mass percentage). Rigorously verified to be distinct from non-target species such as those sourced from yaks or camels.No cross-reactivity, ensuring every judgment is precise and error-free.

Dual-channel error prevention mechanism:Utilizes a dual-color fluorescence probe (FAM channel for ovine target, HEX channel for internal control) with built-in positive and negative controls to effectively monitor PCR inhibitors during extraction, eliminating false-negative results.

Excellent lid stability:The reagent demonstrates strong resistance to environmental interference. The liquid components remain stable with no significant performance degradation after being exposed at room temperature for up to 8 hours, making it ideal for bulk aliquoting and high-throughput processing of large sample volumes.

Use Cases

Sample type:Fresh and cooked meats, food products, cosmetics, and animal feed.

Compatible Instruments (Open Platform):Widely compatible with mainstream real-time PCR instruments on the market (e.g., Bio-Rad CFX96, ABI StepOne Plus / Q3, and Labtech MA-6000).

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