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Tomato Ringspot Virus (ToRSV) RNA Nucleic Acid Test Kit

Quantitative Real-Time PCRHigh Sensitivity

PCR DETECTION KIT

Tomato Ringspot Virus (ToRSV) RNA Nucleic Acid Test Kit

Name: 番茄环斑病毒（ToRSV）RNA核酸检测试剂盒
Brand: 西诺通科
SKU: 48T/盒

10 copies

Limit of Detection

40-45

Cyclic number

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Citation Standards

GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method

SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method

ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms

Technical Principles

Real-time PCR

Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.

Nucleic Acid Extraction

Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.

PCR amplification

Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.

Fluorescence Detection

The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.

Core Performance Metrics

Core Technical Specifications

Rigorous quality control system ensures stability and reliability for every product batch.

Limit of Detection (LOD)

30 copies/reaction

Specificity

No cross-reactivity

Linear Range

10^1 - 10^8 copies/mL

Repeatability (CV)

≤3%

Reaction System

25 μL / 50 μL

Packaging Size

48T/box

Amplification Procedure

Standard Amplification Procedure

Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.

Steps	Temperature	Time	Cyclic Number	Description
pre-deformation	95°C	3-5 min	1	Activate DNA polymerase
gender transition	95°C	10-15 sec	40-45	Collect fluorescence signal
Annealing/Extension	55-60°C	30-40 sec	40-45	Collect fluorescence signal

Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.

Compatible Instruments

Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs

ABI 7500 / 7500 Fast

LightCycler 480 II

CFX96 / CFX384

QuantStudio 5 / 6 / 7

Mx3000P / Mx3500P

Rotor-Gene Q

Storage Conditions

Reagent Storage

Store below 20°C in the dark. Valid for 12 months.

Shipping Conditions

Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.

Storage after reconstitution

2-8°C for stable storage of 7 days; long-term storage at -20°C

Important Notes

Not intended for clinical diagnosis.

Wear a lab coat and gloves during operation to prevent contamination.

Vortex all reagents thoroughly and briefly centrifuge prior to use.

Prepare the reaction mixture in a laminar flow hood or biosafety cabinet.

Product Details

Product Overview

High-efficiency qPCR assay for detection of Tomato ringspot virus (ToRSV). This kit uses TaqMan probe technology (FAM channel) to directly target the viral CP gene. Compared to conventional PCR, it offers a lower limit of detection, faster turnaround time, and fully closed-tube processing to significantly reduce the risk of aerosol contamination.

Core Advantages

Top choice for quarantine: Perfectly aligned with SN/T 3398-2012 standard for multiplex detection of soybean quarantine viruses.

High Sensitivity: Detects viruses even during the incubation period or with minimal infection due to an extremely low detection limit.

Safety: Closed-tube detection eliminates post-electrophoresis steps and toxic dyes like EB.

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