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黄牛-derived component nucleic acid detection kit

Quantitative Real-Time PCRHigh Sensitivity

PCR DETECTION KIT

黄牛-derived component nucleic acid detection kit

In the regulatory oversight of premium beef and related products, distinguishing authentic "cattle" (Bos taurus) from low-cost "buffalo" (Bubalus bubalis) is critical to preventing food fraud. This kit employs real-time quantitative PCR (TaqMan probe technology), specifically targeting the bovine Cytb gene. Developed in strict compliance with GB/T 38164-2019, this product delivers highly accurate species identification for food safety authorities, third-party laboratories, and high-end beef processing enterprises.

10 copies

Limit of Detection

40-45

Cyclic number

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Citation Standards

GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method

SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method

ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms

Technical Principles

Real-time PCR

Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.

Nucleic Acid Extraction

Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.

PCR amplification

Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.

Fluorescence Detection

The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.

Core Performance Metrics

Core Technical Specifications

Rigorous quality control system ensures stability and reliability for every product batch.

Limit of Detection (LOD)

0.1% (by mass)

Specificity

No cross-reactivity

Linear Range

10^1 - 10^8 copies/mL

Repeatability (CV)

≤3%

Reaction System

25 μL / 50 μL

Packaging Size

48T/box

Amplification Procedure

Standard Amplification Procedure

Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.

Steps	Temperature	Time	Cyclic Number	Description
pre-deformation	95°C	3-5 min	1	Activate DNA polymerase
gender transition	95°C	10-15 sec	40-45	Collect fluorescence signal
Annealing/Extension	55-60°C	30-40 sec	40-45	Collect fluorescence signal

Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.

Compatible Instruments

Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs

ABI 7500 / 7500 Fast

LightCycler 480 II

CFX96 / CFX384

QuantStudio 5 / 6 / 7

Mx3000P / Mx3500P

Rotor-Gene Q

Storage Conditions

Reagent Storage

Store below 20°C in the dark. Valid for 12 months.

Shipping Conditions

Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.

Storage after reconstitution

2-8°C for stable storage of 7 days; long-term storage at -20°C

Important Notes

Not intended for clinical diagnosis.

Wear a lab coat and gloves during operation to prevent contamination.

Vortex all reagents thoroughly and briefly centrifuge prior to use.

Prepare the reaction mixture in a laminar flow hood or biosafety cabinet.

Product Details

Core Advantages

Extreme specificity, precise breed differentiation:The probe design is highly specific and rigorously validated for specificity.No cross-reactivity with buffalo, camel, goat, or sheep sources.Effectively detects adulteration where water buffalo meat is substituted for yellow beef.

Authoritative National Standard Backing:Developed and manufactured in strict accordance with national standard GB/T 38164-2019, with a limit of detection (LOD) reaching1%(weight percent), fully meeting compliance requirements for statutory testing agencies.

Built-in quality control to eliminate false positives and negatives:The kit includes bovine-derived positive and negative controls as standard, enabling comprehensive quality control by laboratory personnel to ensure test results are accurate, scientific, and reliable.

Unbeatable lid-opening stability:The liquid reagent components remain stable with no significant loss in amplification performance after being opened and exposed at room temperature for up to 8 hours, greatly simplifying bulk reagent preparation and instrument loading for large-scale sample processing.

Use Cases

Sample type:Fresh meat and meat products (e.g., beef rolls, beef jerky, beef balls), offal, dairy products, and animal feed.

Compatible Instruments (Open Platform):Widely compatible with mainstream real-time PCR instruments featuring FAM fluorescence channels (e.g., Bio-Rad CFX96, ABI StepOne Plus / Q3, Yirui MA-6000).

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