单核细胞增生李斯特氏菌（Listeria monocytogenes）是极具危害性的食源性致病菌之一，对食品加工与公共卫生安全构成重大威胁。本试剂盒基于实时荧光定量PCR（TaqMan探针法）技术，精准靶向其特异性的 hly 保守基因。本产品全面贴合最新发布的 GB 4789.30-2025 食品安全国家标准，为食品厂放行、环境监控及致病菌溯源提供快速、权威的分子级诊断方案。
Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.
Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.
Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.
The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.
Rigorous quality control system ensures stability and reliability for every product batch.
Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.
| Steps | 温度 | Time | Cyclic Number | Description |
|---|---|---|---|---|
| pre-deformation | 95°C | 3-5 min | 1 | Activate DNA polymerase |
| gender transition | 95°C | 10-15 sec | 40-45 | Collect fluorescence signal |
| Annealing/Extension | 55-60°C | 30-40 sec |
Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.
Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs
符合国标要求：严格参照GB 4789.30-2016（食品安全国家标准 食品微生物学检验 单核细胞增生李斯特氏菌检验）及SN/T 1870-2016（进出口食品中食源性致病菌检测方法 实时荧光PCR法）开发，检测流程与标准方法无缝衔接。
灵敏度高：检出限达1 CFU/25g，可有效检测低浓度致病菌污染。
特异性强：引物探针基于单核细胞增生李斯特氏菌特异性基因（hlyA）的保守区域设计，经验证与常见食源性致病菌无交叉反应。
UNG酶防污染体系：引入UNG酶（尿嘧啶-N-糖基化酶）防污染系统，有效消除PCR产物的气溶胶污染，降低假阳性风险。
酶原料自产：所用关键酶（热启动Taq酶、UNG酶等）均为自有技术平台自主研发生产，批间差小、活性稳定，从源头保障试剂品质。
A professional technical team providing comprehensive product application and testing solution support.
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