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Quantitative Real-Time PCRHigh Sensitivity

PCR DETECTION KIT

NPTII Gene Nucleic Acid Test Kit

Name: NPTII 基因核酸检测试剂盒
Brand: 西诺通科
SKU: 48T/盒

The NPTII gene is one of the most widely used selectable marker genes in global transgenic crop development and serves as a "core target" for detecting GMO content in food and agricultural products. This kit, based on real-time quantitative PCR (TaqMan probe technology), is designed specifically for qualitative screening of transgenic components in plants and their deep-processed derivatives. Developed in strict compliance with multiple national and industry standards for GMO detection, this product provides highly credible molecular biology evidence for identification.

10 copies

Limit of Detection

40-45

Cyclic number

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Citation Standards

GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method

SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method

ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms

Technical Principles

Real-time PCR

Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.

Nucleic Acid Extraction

Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.

PCR amplification

Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.

Fluorescence Detection

The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.

Core Performance Metrics

Core Technical Specifications

Rigorous quality control system ensures stability and reliability for every product batch.

Limit of Detection (LOD)

0.01%

Specificity

No cross-reactivity

Linear Range

10^1 - 10^8 copies/mL

Repeatability (CV)

≤3%

Reaction System

25 μL / 50 μL

Packaging Size

48T/box

Amplification Procedure

Standard Amplification Procedure

Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.

Steps	Temperature	Time	Cyclic Number	Description
pre-deformation	95°C	3-5 min	1	Activate DNA polymerase
gender transition	95°C	10-15 sec	40-45	Collect fluorescence signal
Annealing/Extension	55-60°C	30-40 sec	40-45	Collect fluorescence signal

Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.

Compatible Instruments

Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs

ABI 7500 / 7500 Fast

LightCycler 480 II

CFX96 / CFX384

QuantStudio 5 / 6 / 7

Mx3000P / Mx3500P

Rotor-Gene Q

Storage Conditions

Reagent Storage

Store below 20°C in the dark. Valid for 12 months.

Shipping Conditions

Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.

Storage after reconstitution

2-8°C for stable storage of 7 days; long-term storage at -20°C

Important Notes

Not intended for clinical diagnosis.

Wear a lab coat and gloves during operation to prevent contamination.

Vortex all reagents thoroughly and briefly centrifuge prior to use.

Prepare the reaction mixture in a laminar flow hood or biosafety cabinet.

Product Details

Core Advantages

Complies with national standards: Developed in strict accordance with Ministry of Agriculture Announcement No. 1782 (2-2012, Qualitative PCR Methods for Markers NPTII, HPT, and PMI in Transgenic Plants and Their Products) and SN/T 1204-2003, among others.

High Sensitivity: Detection limit of 0.1% (mass fraction) or 100 copies/reaction, enabling effective detection of trace GMO components. High Specificity: Primers and probes designed based on conserved regions of the NPTII gene; verified to have no cross-reactivity with common genetic elements.

In-house enzyme production: Key enzymes (e.g., HotStart Taq polymerase, UNG) are developed and manufactured using our proprietary platform, ensuring low batch-to-batch variability and stable activity.

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