Product Overview
本产品专为精准检测番茄斑萎病毒（TSWV）设计。采用实时荧光定量PCR技术，特异性靶向病毒NP基因，结合TaqMan探针（VIC通道），可实现对植物样本中微量病毒核酸的高灵敏度定性分析，是农业及检验检疫部门进行病害排查的理想工具。
Core Advantages
合规可靠：严格遵循 GB/T 28982-2012 国家标准检测方法。
抗干扰强：高特异性引物探针，与其他常见植物病毒无交叉反应。
适用广泛：完美适配各类种子、苗木及无性繁殖活体植物材料。
Current template:PCR Kit Template
Quantitative Real-Time PCR高灵敏度
PCR DETECTION KIT
番茄斑萎病毒（TSWV）RNA核酸检测试剂盒
10 copies
Limit of Detection
40-45
Cyclic number
Citation Standards
GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method
SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method
ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms
Technical Principles
Real-time PCR
Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.
Nucleic Acid Extraction
Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.
PCR amplification
Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.
Fluorescence Detection
The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.
Core Performance Metrics
Core Technical Specifications
Rigorous quality control system ensures stability and reliability for every product batch.
Limit of Detection (LOD)
30 copies/reaction
Specificity
No cross-reactivity
Linear Range
10^1 - 10^8 copies/mL
Repeatability (CV)
≤3%
Reaction System
25 μL / 50 μL
Packaging Size
48T/box
Amplification Procedure
Standard Amplification Procedure
Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.
| Steps | 温度 | Time | Cyclic Number | Description |
|---|---|---|---|---|
| pre-deformation | 95°C | 3-5 min | 1 | Activate DNA polymerase |
| gender transition | 95°C | 10-15 sec | 40-45 | Collect fluorescence signal |
| Annealing/Extension | 55-60°C | 30-40 sec |
Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.
Compatible Instruments
Compatible Instruments
Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs
ABI 7500 / 7500 Fast
LightCycler 480 II
CFX96 / CFX384
QuantStudio 5 / 6 / 7
Mx3000P / Mx3500P
Rotor-Gene Q
Storage Conditions
Reagent Storage
Store below 20°C in the dark. Valid for 12 months.
Shipping Conditions
Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.
Storage after reconstitution
2-8°C for stable storage of 7 days; long-term storage at -20°C
Important Notes
Not intended for clinical diagnosis.
Wear a lab coat and gloves during operation to prevent contamination.
Vortex all reagents thoroughly and briefly centrifuge prior to use.
请在超净工作台或生物安全柜中配制反应体系
Product Details
Product Details
Downloads & Support
Downloads & Technical Support
A professional technical team providing comprehensive product application and testing solution support.
Need technical consultation?
Our technical expert team will provide you with professional product consulting and customized solutions.
Get Technical Consultation Now