Product Overview
本产品基于经典且经济的常规RT-PCR技术开发，通过特异性引物对番茄环斑病毒（ToRSV）进行扩增，配合琼脂糖凝胶电泳（扩增产物449 bp）即可直观判定结果。适合具备基础分子生物学操作条件的实验室进行常规初筛与检疫鉴定。
Core Advantages
经典标准：符合 SN/T 2670-2010 出入境检验检疫行业标准。
成本效益高：无需昂贵的荧光定量PCR设备即可完成精准定性检测。
结果直观：电泳条带清晰，结果判定标准明确。
Current template:PCR Kit Template
Quantitative Real-Time PCR高灵敏度
PCR DETECTION KIT
番茄环斑病毒（ToRSV）RNA核酸检测试剂盒（RT-PCR法）
10 copies
Limit of Detection
40-45
Cyclic number
Citation Standards
GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method
SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method
ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms
Technical Principles
Real-time PCR
Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.
Nucleic Acid Extraction
Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.
PCR amplification
Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.
Fluorescence Detection
The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.
Core Performance Metrics
核心技术指标
Rigorous quality control system ensures stability and reliability for every product batch.
Limit of Detection (LOD)
/
Specificity
No cross-reactivity
Linear Range
10^1 - 10^8 copies/mL
Repeatability (CV)
≤3%
Reaction System
25 μL / 50 μL
Packaging Size
48T/box
Amplification Procedure
Standard Amplification Procedure
Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.
| Steps | 温度 | Time | Cyclic Number | Description |
|---|---|---|---|---|
| pre-deformation | 95°C | 3-5 min | 1 | Activate DNA polymerase |
| 变性 | 95°C | 10-15 sec | 40-45 | Collect fluorescence signal |
| Annealing/Extension | 55-60°C | 30-40 sec |
Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.
Compatible Instruments
Compatible Instruments
Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs
ABI 7500 / 7500 Fast
LightCycler 480 II
CFX96 / CFX384
QuantStudio 5 / 6 / 7
Mx3000P / Mx3500P
Rotor-Gene Q
Storage Conditions
Reagent Storage
Store below 20°C in the dark. Valid for 12 months.
Shipping Conditions
Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.
Storage after reconstitution
2-8°C for stable storage of 7 days; long-term storage at -20°C
Important Notes
Not intended for clinical diagnosis.
Wear a lab coat and gloves during operation to prevent contamination.
Vortex all reagents thoroughly and briefly centrifuge prior to use.
请在超净工作台或生物安全柜中配制反应体系
Product Details
Product Details
Downloads & Support
Downloads & Technical Support
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