Current template:PCR Kit Template

Listeria monocytogenes Nucleic Acid Test Kit -test1

Quantitative Real-Time PCRHigh Sensitivity

PCR DETECTION KIT

Listeria monocytogenes Nucleic Acid Test Kit -test1

Name: 单核细胞增生李斯特氏菌核酸检测试剂盒-test1
Brand: 西诺通科

Listeria monocytogenes is a highly dangerous foodborne pathogen posing significant threats to food processing and public health safety. This kit utilizes real-time quantitative PCR (TaqMan probe method) to precisely target the specific hly conserved gene. Fully compliant with the latest GB 4789.30-2025 National Food Safety Standards, this product delivers a rapid, authoritative molecular diagnostic solution for food plant release testing, environmental monitoring, and pathogen tracing.

10 copies

Limit of Detection

40-45

Cyclic number

Get in Touch

Citation Standards

GB/T 27405-2008National Food Safety Standard: Microbiological Examination of Foods — PCR Detection Method

SN/T 1193-2019Nucleic Acid Detection Methods for Foodborne Pathogens in Imported and Exported Foods: Real-Time Fluorescent PCR Method

ISO 22174:2005Microbiology of food and feed — PCR method for detecting genetically modified organisms

Technical Principles

Real-time PCR

Enzymatic reaction based on DNA polymerase that uses real-time fluorescence monitoring of PCR amplification to achieve qualitative or quantitative detection of target nucleic acids.

Nucleic Acid Extraction

Extract target DNA/RNA from samples and remove inhibitors such as proteins and polysaccharides to obtain high-purity nucleic acid templates for PCR.

PCR amplification

Under the guidance of specific primers and fluorescent probes, DNA polymerase catalyzes dNTP incorporation to synthesize a new strand, releasing one fluorescent signal per amplification cycle.

Fluorescence Detection

The instrument acquires fluorescence signals in real time and performs precise quantification of target nucleic acids by comparing Ct values against a standard curve.

Core Performance Metrics

Core Technical Specifications

Rigorous quality control system ensures stability and reliability for every product batch.

Limit of Detection (LOD)

Minimum 10 copies/reaction

Specificity

No cross-reactivity

Linear Range

10^1 - 10^8 copies/mL

Repeatability (CV)

≤3%

Reaction System

25 μL / 50 μL

Packaging Size

48TB / 96TB

Amplification Procedure

Standard Amplification Procedure

Optimized reaction conditions to achieve the best balance between amplification efficiency and specificity.

Steps	Temperature	Time	Cyclic Number	Description
pre-deformation	95°C	3-5 min	1	Activate DNA polymerase
gender transition	95°C	10-15 sec	40-45	Collect fluorescence signal
Annealing/Extension	55-60°C	30-40 sec	40-45	Collect fluorescence signal

Note: Specific reaction conditions may vary depending on the instrument model and target analyte. Please refer to the product manual or consult a technical expert.

Compatible Instruments

Widely compatible with mainstream real-time PCR instruments to meet diverse laboratory equipment needs

ABI 7500 / 7500 Fast

LightCycler 480 II

CFX96 / CFX384

QuantStudio 5 / 6 / 7

Mx3000P / Mx3500P

Rotor-Gene Q

Storage Conditions

Reagent Storage

Store below 20°C in the dark. Valid for 12 months.

Shipping Conditions

Low-temperature cold chain transport to prevent repeated freeze-thaw cycles.

Storage after reconstitution

2-8°C for stable storage of 7 days; long-term storage at -20°C

Important Notes

Not intended for clinical diagnosis.

Wear a lab coat and gloves during operation to prevent contamination.

Vortex all reagents thoroughly and briefly centrifuge prior to use.

Prepare the reaction mixture in a laminar flow hood or biosafety cabinet.

Product Details

Key Advantages Compliance with National Standards: Developed in strict accordance with GB 4789.30-2016 (National Food Safety Standard—Microbiological Examination of Food—Detection of Listeria monocytogenes) and SN/T 1870-2016 (Methods for Detection of Foodborne Pathogens in Imported and Exported Foods—Real-time Fluorescence PCR), ensuring seamless integration with standard testing procedures. High Sensitivity: Achieves a limit of detection as low as 1 CFU/25g, enabling effective detection of low-concentration pathogen contamination. Strong Specificity: Primers and probes are designed based on conserved regions of the hlyA gene specific to Listeria monocytogenes. Validated to show no cross-reactivity with common foodborne pathogens. UNG Enzyme Anti-Contamination System: Incorporates an UNG enzyme (Uracil-N-Glycosylase) system to eliminate aerosol contamination from PCR products, significantly reducing the risk of false positives. In-house Enzyme Production: Key enzymes used (including Hot-start Taq polymerase and UNG enzyme) are independently developed and manufactured using our proprietary technology platform. This ensures minimal batch-to-batch variation, stable activity, and guaranteed reagent quality from the source.

Downloads & Support

Downloads & Technical Support

A professional technical team providing comprehensive product application and testing solution support.

Need technical consultation?

Our technical expert team will provide you with professional product consulting and customized solutions.

Get Technical Consultation Now